需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Identification of Functional Protein Regions Through Chimeric Protein Construction
Chapters
- 00:04Title
- 00:49Chimeric Protein Design
- 05:00Preparation for Molecular Cloning
- 07:05Polymerase Chain Reaction (PCR) Amplification of the Individual DNA Fragments Forming the Chimera and to Generate the Chimeric DNA Sequence
- 09:22Results: Generation of the Oncostatin M BC Loop Chimera
- 10:55Conclusion
Structurally related proteins frequently exert distinct biological functions. The exchange of equivalent regions of these proteins in order to create chimeric proteins constitutes an innovative approach to identify critical protein regions that are responsible for their functional divergence.
