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Chapters
- 00:04Title
- 00:48General Lymantria dispar (LD652) Cell and Virus Culture
- 01:33General Viral Infection Protocol for Luminescence-based VSV rescue Assays in LD652 Cells
- 02:43Luciferase Assay
- 04:06Results: Coinfection with VSV-LUC with VACV-WR Results in More LUC Signals
- 05:04Conclusion
Her presenterer vi protokoller for å identifisere 1) virus-kodede immunomodulators som fremmer arbovirus replikering og 2) eukaryote vert faktorer som begrenser arbovirus replikering. Metodene fluorescens og luminescence-baserte tillate forskere å raskt få kvantitative readouts arbovirus replikasjon i enkle analyser med lav signal-til-støy-forhold.
