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Immunology and Infection

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JoVE Journal Immunology and Infection
An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells
 

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

Article DOI: 10.3791/58118-v • 08:39 min • August 22nd, 2018
August 22nd, 2018 •
1, 1, 2, 1,2,3
1LamImSys Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 2LamImSys Lab, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 3CIBER de Enfermedades Cardiovasculares

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Here, we present a protocol for the in vivo determination of naïve CD4 T cell (T cell) activation, proliferation, and Th1 differentiation induced by GM-CSF bone marrow (BM)-derived dendritic cells (DCs). In addition, this protocol describes BM and T-cell isolation, DC generation, and DC and T-cell adoptive transfer.

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In Vivo Mouse Model Naïve CD4 T Cell Activation Proliferation Th1 Differentiation Bone Marrow-derived Dendritic Cells Immunology Antigen Presenting Cell Adaptive Immunity Cell Manipulation Bone Marrow Cell Isolation Hind Limbs Skin Incision Muscle Separation Bone Disarticulation Petri Dish Complete Medium Bone Flushing
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