Adenoviral gene transfer into naive CD4 T cells with transgenic expression of the Coxsackie adenovirus receptor enables the molecular analysis of regulatory T cell differentiation in vitro.
Warth, S. C., Heissmeyer, V. Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation. J. Vis. Exp. (78), e50455, doi:10.3791/50455 (2013).