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体内高分子量截离法从脑间质液中提取大细胞外蛋白的微透析方法
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In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
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体内高分子量截离法从脑间质液中提取大细胞外蛋白的微透析方法

14,168 Views

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08:17 min

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September 26, 2018

DOI:

10.3791/57869-v

DOI:
10.3791/57869-v

•

08:17 min
September 26, 2018

•

7 Views
1Department of Neuropathology, Graduate School of Medicine,University of Tokyo

Summary

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体内微透析已使大脑间质液中存在的分子从清醒的、自由行为的动物中收集。为了分析该化合物中相对较大的分子, 本文重点研究了用高分子量切断膜探针的微透析协议。

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Yamada, K. In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes. J. Vis. Exp. (139), e57869, doi:10.3791/57869 (2018).

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