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线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定

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生物化学

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JoVE 杂志生物化学

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

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线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定

DOI:

10.3791/57807-v

•

09:35 min

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October 15, 2018

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Sumino Yanase², Kayo Yasuda, Naoaki Ishii

¹Department of Health Science, School of Sports & Health Science,Daito Bunka University, ²Department of Molecular Life Science, School of Medicine,Tokai University, ³Department of Health Management, School of Health Study,Tokai University

Chapters

00:04Title
00:37Caenorhabditis elegans Synchronized Culture
03:21Extraction of Cellular Fraction from C. elegans
05:35Lactate, Pyruvate and Protein Assays
07:32Results: Efficiency of Lactate and Pyruvate Colorimetric Assays After Protein Precipitation of C. elegans
08:26Conclusion

Summary

自动翻译

本文介绍了一种改良的小尺度萃取和比色法测定乳酸和丙酮酸在线虫中的含量.使用商用试剂盒时, 其灵敏度和准确度的技术发展非常重要。在萃取中蛋白质沉淀是定量测定细胞内代谢产物的关键步骤。

Tags

Colorimetric Assay Lactate Pyruvate Caenorhabditis Elegans Nematode Protein Precipitation Homogenization E. Coli OP50 NGM Agar Plates Gravid Hermaphrodites Egg Isolation L1 Larvae Synchronous Culture Colorimetric Analysis Biochemical Methodology

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