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小規模な比色試金の細胞内乳酸と線虫の線虫のピルビン酸

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生物化学

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JoVE 杂志生物化学

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

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小規模な比色試金の細胞内乳酸と線虫の線虫のピルビン酸

6,787 Views

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09:35 min

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October 15, 2018

DOI:

10.3791/57807-v

DOI:

10.3791/57807-v

•

09:35 min

October 15, 2018

•

16 Views

Sumino Yanase^1,2, Kayo Yasuda³, Naoaki Ishii³

¹Department of Health Science, School of Sports & Health Science,Daito Bunka University, ²Department of Molecular Life Science, School of Medicine,Tokai University, ³Department of Health Management, School of Health Study,Tokai University

Summary

変更された小規模な抽出と乳酸と線虫のピルビン酸の比色定量法について述べる線虫。商業アッセイキットを利用するときの感度と精度の技術の開発が重要です。抽出の蛋白質の沈殿物は、細胞内の代謝物の定量法の最も重要なステップです。

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