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线粒体钙在分离线粒体和培养细胞中的流入分析
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生物化学
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JoVE 杂志 生物化学
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
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线粒体钙在分离线粒体和培养细胞中的流入分析

13,489 Views

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08:29 min

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April 27, 2018

DOI:

10.3791/57225-v

DOI:
10.3791/57225-v

•

08:29 min
April 27, 2018

•

4 Views
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1Department of Pediatrics,Emory University School of Medicine, 2Emory College of Arts and Sciences,Emory University

Tags

Mitochondrial Calcium InfluxCalcium SignalingMitochondrial Calcium DynamicsDisease ConditionsPlate-reader-based Mitochondrial Calcium UptakeConfocal-microscope-based Mitochondrial Calcium InfluxIsolated HeartMSEGTA BufferCalcium Green-5NCalcium Chloride

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Maxwell, J. T., Tsai, C., Mohiuddin, T. A., Kwong, J. Q. Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells. J. Vis. Exp. (134), e57225, doi:10.3791/57225 (2018).

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