Journal
/
发育生物学
/
Medición en tiempo real de la permeabilidad de la barrera epitelial en organoides Intestinal humano
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
Please note that all translations are automatically generated. Click here for the English version.

Medición en tiempo real de la permeabilidad de la barrera epitelial en organoides Intestinal humano

13,728 Views

•

08:04 min

•

December 18, 2017

DOI:

10.3791/56960-v

DOI:
10.3791/56960-v

•

08:04 min
December 18, 2017

•

4 Views
, , , ,
1Department of Internal Medicine, Division of Gastroenterology,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease,University of Michigan

Summary

Automatically generated

Este protocolo describe la medición de la permeabilidad de la barrera epitelial en el tratamiento farmacológico siguiente en tiempo real en humanos organitas intestinal mediante microscopía fluorescente y vivo microscopia celular.

Explore More Videos

Epithelial Barrier PermeabilityHuman Intestinal OrganoidsReal-time MeasurementFITC-dextranMicroinjectionGastroenterologyToxinsBacterial ProductsPharmacologic TreatmentsVisualizationQuantitative Measure

Read Article

Cite this Article

Hill, D. R., Huang, S., Tsai, Y., Spence, J. R., Young, V. B. Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids. J. Vis. Exp. (130), e56960, doi:10.3791/56960 (2017).

Download .ris file

Copy

Share Video

.

Copy