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发育生物学
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人間の腸 Organoids 上皮関門の透過性のリアルタイム計測
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
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人間の腸 Organoids 上皮関門の透過性のリアルタイム計測

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08:04 min

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December 18, 2017

DOI:

10.3791/56960-v

DOI:
10.3791/56960-v

•

08:04 min
December 18, 2017

•

4 Views
, , , ,
1Department of Internal Medicine, Division of Gastroenterology,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease,University of Michigan

Summary

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このプロトコルは、蛍光顕微鏡を用いたヒトの腸オルガノイドのリアルタイム次の薬理学的治療における上皮バリア透過性の測定について説明し、細胞顕微鏡検査をライブします。

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Cite this Article

Hill, D. R., Huang, S., Tsai, Y., Spence, J. R., Young, V. B. Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids. J. Vis. Exp. (130), e56960, doi:10.3791/56960 (2017).

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