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Genome-wide kvantifiering av översättning i spirande jäst av Ribosomen profilering
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生物化学
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JoVE 杂志 生物化学
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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Genome-wide kvantifiering av översättning i spirande jäst av Ribosomen profilering

11,247 Views

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12:57 min

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December 21, 2017

DOI:

10.3791/56820-v

DOI:
10.3791/56820-v

•

12:57 min
December 21, 2017

•

2 Views
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1Department of Dermatology,Boston University School of Medicine

Tags

Ribosome ProfilingGenome-wideTranslationBudding YeastSaccharomyces CerevisiaeProtein TranslationTranslational RegulationCell LysatePoly(A) MRNASucrose GradientRNase ICycloheximide

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Beaupere, C., Chen, R. B., Pelosi, W., Labunskyy, V. M. Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling. J. Vis. Exp. (130), e56820, doi:10.3791/56820 (2017).

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