Journal
/
发育生物学
/
人成肌细胞的分离，造血分化的评估和存储操作的钙进入测量
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Please note that all translations are automatically generated. Click here for the English version.

人成肌细胞的分离，造血分化的评估和存储操作的钙进入测量

9,834 Views

•

10:45 min

•

July 26, 2017

DOI:

10.3791/55918-v

DOI:
10.3791/55918-v

•

10:45 min
July 26, 2017

•

3 Views
, , ,
1Department of Orthopedic Surgery,Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences,Geneva Medical Center, 3Department of Cell Physiology and Metabolism,Geneva Medical Center

Summary

Automatically generated

在这里，我们描述了一种从成年肌肉组织中获得纯种人成肌细胞的方法。这些细胞用于研究体外骨骼肌分化，特别是研究参与Ca 2+信号传导的蛋白质。

Explore More Videos

Human MyoblastsMyogenic DifferentiationStore-operated Calcium EntrySkeletal MuscleMuscle RegenerationIonic ChannelsTranscription FactorsMuscular DystrophiesTrypsinCell CultureCell IsolationCell Staining

Read Article

Cite this Article

Laumonier, T., Koenig, S., Saüc, S., Frieden, M. Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement. J. Vis. Exp. (125), e55918, doi:10.3791/55918 (2017).

Download .ris file

Copy

Share Video

.

Copy