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JoVE Journal Neuroscience
Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations
 

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Article DOI: 10.3791/55133-v • 12:27 min • February 15th, 2017
February 15th, 2017 •
1, 1
1Physiology & Neurobiology, Geisel School of Medicine at Dartmouth

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This protocol describes large-scale reconstructions of selective neuronal populations, labeled following retrograde infection with a modified rabies virus expressing fluorescent markers, and independent, unbiased cluster analyses that enable comprehensive characterization of morphological metrics among distinct neuronal subclasses.

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Keywords: Neuron Reconstruction Morphological Metrics Unbiased Clustering Neuroanatomical Data Analysis EGFP-labeled Neurons Primate Brain Tissue Dendritic Arborization Neuronal Subclasses
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