Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations
Bölümler
- 00:05Başlık
- 01:15Neuronal Reconstruction: Cell Body
- 04:25Neuronal Reconstruction: Dendritic Arbors
- 08:29Independent Clustering
- 09:24Results: Independent Clustering of 160 TRN Neurons
- 11:35Conclusion
This protocol describes large-scale reconstructions of selective neuronal populations, labeled following retrograde infection with a modified rabies virus expressing fluorescent markers, and independent, unbiased cluster analyses that enable comprehensive characterization of morphological metrics among distinct neuronal subclasses.
