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Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
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发育生物学
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JoVE 杂志 发育生物学
Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

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08:01 min

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April 19, 2018

DOI:

10.3791/57702-v

DOI:
10.3791/57702-v

•

08:01 min
April 19, 2018

•

2 Views
,
1Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology,Monash University

Summary

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We present an automated method for three-dimensional reconstruction of the Caenorhabditis elegans germline. Our method determines the number and position of each nucleus within the germline and analyses germline protein distribution and cytoskeletal structure.

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Gopal, S., Pocock, R. Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton. J. Vis. Exp. (134), e57702, doi:10.3791/57702 (2018).

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