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Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
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JoVE 杂志 免疫与感染
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance

DOI:
10.3791/54653-v

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10:55 min

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October 16, 2018

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, , , ,
1Clinical Microbiology, Department of Translational Medicine,Lund University, 2Department of Molecular Biology,Umea University

Chapters

  • 00:04Title
  • 00:49Detection of Vitronectin (Vn) Binding at the Bacterial Surface Using Flow Cytometry
  • 02:18ELISA Analysis of the Interaction Between Recombinant Protein H (PH) and Vn
  • 04:11Study of the Interaction Kinetics of Recombinant PH and Vn Using Bio-layer Interferometry
  • 04:33Bacterial Adherence to the Vn-coated Glass Surfaces
  • 05:32Vn-dependent Adherence of Bacteria to Epithelial Cells
  • 07:29Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum
  • 08:29Results: Interaction of Vitronectin and Bacteria
  • 10:10Conclusion

Summary

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This report describes protocols for characterizing interactions between bacterial outer membrane proteins and the human complement regulator vitronectin. The protocols can be used to study the binding reactions and biological function of vitronectin in any bacterial species.

Tags

VitronectinBacterial AdhesionSerum ResistanceHost-pathogen InteractionComplement InhibitorFlow CytometryELISAAntihuman Vitronectin AntibodyFITC-conjugated AntibodyRecombinant PH Protein

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