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JoVE Journal Neuroscience
Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay
 

Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay

Article DOI: 10.3791/54443-v • 09:49 min • September 6th, 2016
September 6th, 2016 •
*1, *2, 1, 1,2
1Center for Advanced Biotechnology and Medicine, Rutgers University, 2Department of Neuroscience and Cell Biology, Rutgers University
* These authors contributed equally

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Methods to accurately measure retinoic acid (RA) levels in small amounts of tissue do not exist. This protocol describes the easy, quantitative measurement of relative RA levels in E8.5 embryos and neurospheres using an RA reporter cell line.

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Keywords: Quantitative Measurement Relative Retinoic Acid Levels E8.5 Embryos Neurosphere Cultures F9 RARE-LacZ Cell-based Reporter Assay RA Signaling Pathway RA Metabolic Enzymes RA Concentration LacZ Staining Gelatin-coated Plates Cell Culture Colony Selection Cell Expansion
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