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Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

24,387 Views

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11:54 min

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January 11, 2011

DOI:

10.3791/2457-v

DOI:
10.3791/2457-v

•

11:54 min
January 11, 2011

•

22 Views
, , , ,
1Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,The University of Florida

Summary

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This video protocol demonstrates the application of the neurosphere assay for the isolation and expansion of neural stem cells from the ganglionic eminences of embryonic day 14-mouse brain.

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Embryonic Mouse Neural Stem Cell CultureNeurosphere AssayStem CellsUndifferentiated CellsCell GenesisAging ProcessInjury And DiseaseNeural Stem Cells (NSCs)Serum-free Culture SystemIsolation And Expansion Of NSCsEmbryonic CNSIn Vitro Expanded NSCsCNS Cell TypesReliable And Consistent ResultsE14 Mouse EmbryosBrain MicrodissectionGanglionic EminencesSingle Cell SuspensionNSA CulturePrimary Neurospheres

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Azari, H., Sharififar, S., Rahman, M., Ansari, S., Reynolds, B. A. Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay. J. Vis. Exp. (47), e2457, doi:10.3791/2457 (2011).

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