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Journal / Developmental Biology / Culturing Human Pluripotent and Neural Stem Cells …
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Developmental Biology

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JoVE Journal Developmental Biology
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
 

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

Article DOI: 10.3791/53685-v • 08:05 min • June 10th, 2016
June 10th, 2016 •
1, 1, 1, 1, 1, 1
1National Human Neural Stem Cell Resource, Childrens Hospital of Orange County Research Institute

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Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.

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Keywords: Human Pluripotent Stem Cells Neural Stem Cells Enclosed Cell Culture System Basic Research Preclinical Research Cell Culture Parameters Multipotent Stem Cells Pluripotent Stem Cells Oxygen Carbon Dioxide Temperature Incubator Process Chamber Buffer Chamber Laminar Hood Sterility Cell Culture Procedure
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