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Verwendung von isolierten Mitochondrien aus minimalen Mengen an Maus-Skelettmuskel für die Hochdurchsatz-Mikroplatten-Atemmessungen
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
DOI:

10:39 min

October 30, 2015

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Chapters

  • 00:05Title
  • 01:30Setup
  • 02:53Running the Assay
  • 07:45Results: Microplate Based Assay Measures Oxygen Consumption in Skeletal Muscle Mitochodria
  • 10:05Conclusion

Summary

自动翻译

The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.

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