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使用分离线粒体从小鼠骨骼肌进行高通量微孔板呼吸测量最小数量
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
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使用分离线粒体从小鼠骨骼肌进行高通量微孔板呼吸测量最小数量

13,294 Views

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10:39 min

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October 30, 2015

DOI:

10.3791/53216-v

DOI:
10.3791/53216-v

•

10:39 min
October 30, 2015

•

28 Views
, , , , ,
1The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, 2The Metabolic Phenotyping Core,Virginia Tech, 3Seahorse Bioscience

Summary

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The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.

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Key Words:Mitochondrial RespirationSkeletal MuscleMouseMicroplateHigh-throughputIsolated MitochondriaRespiratory Measurements

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Cite this Article

Boutagy, N. E., Rogers, G. W., Pyne, E. S., Ali, M. M., Hulver, M. W., Frisard, M. I. Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements. J. Vis. Exp. (104), e53216, doi:10.3791/53216 (2015).

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