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Utilizzo di mitocondri isolati da minime quantità di muscolo scheletrico del mouse per High micropiastre rendimento respiratorie Misure
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

Utilizzo di mitocondri isolati da minime quantità di muscolo scheletrico del mouse per High micropiastre rendimento respiratorie Misure

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10:39 min

October 30, 2015

DOI:

10:39 min
October 30, 2015

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Summary

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The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.

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