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JoVE Journal Developmental Biology
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
 

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

Article DOI: 10.3791/53202-v • 09:40 min • September 30th, 2015
September 30th, 2015 •
1, 1, 1
1Department of Neuroscience, Biomedical Center, Uppsala University

章节

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Automatic Translation

This protocol describes a method to dissect, experimentally manipulate and culture whole retinal explants from chicken embryos. The explant cultures are useful when high success rate, efficacy and reproducibility are needed to test the effects of plasmids for electroporation and/or reagent substances, i.e., enzymatic inhibitors.

Tags

Keywords: Whole Retinal Explants Chicken Embryos Electroporation Chemical Reagents Experimental Model Central Nervous System Intraocular Injections Ex Vivo Culture Controlled Exposure Rapid Reproducible Cost-effective
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