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In Ovo Electroporation in the Chicken Auditory Brainstem
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发育生物学
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JoVE 杂志 发育生物学
In Ovo Electroporation in the Chicken Auditory Brainstem

In Ovo Electroporation in the Chicken Auditory Brainstem

8,291 Views

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10:14 min

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June 09, 2017

DOI:

10.3791/55628-v

DOI:
10.3791/55628-v

•

10:14 min
June 09, 2017

•

1 Views
, ,
1Roxelyn and Richard Pepper Department of Communication Sciences and Disorders,Northwestern University, 2Knowles Hearing Research Center,Northwestern University, 3Department of Neurobiology,Northwestern University

Summary

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Auditory brainstem neurons of avians and mammals are specialized for fast neural encoding, a fundamental process for normal hearing functions. These neurons arise from distinct precursors of embryonic hindbrain. We present techniques utilizing electroporation to express genes in the hindbrain of chicken embryos to study gene function during auditory development.

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Cite this Article

Lu, T., Cohen, A. L., Sanchez, J. T. In Ovo Electroporation in the Chicken Auditory Brainstem. J. Vis. Exp. (124), e55628, doi:10.3791/55628 (2017).

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