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JoVE Journal Developmental Biology
Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
 

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Article DOI: 10.3791/53162-v • 08:10 min • December 14th, 2015
December 14th, 2015 •
1, 2, 3, 4, 5, 5
1Department of Biomedical Science, The Bateson Centre, University of Sheffield, 2Institute of Genetic Medicine, Newcastle University, 3Department of Cardiovascular Science, The Bateson Centre, University of Sheffield, 4School of Biochemistry, University of Bristol, 5Biology Department, University of York

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The manuscript here provides a simple set of methods for analysing the secretion and diffusion of fluorescently tagged ligands in Xenopus. This provides a context for testing the ability of other proteins to modify ligand distribution and allowing experiments that may give insight into mechanisms regulating morphogen gradients.

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Confocal Analysis Xenopus Laevis Wnt Morphogen Gradient Shh Morphogen Gradient Ligand Distribution GFP-tagged Ligands Synthetic MRNA Injection Lineage Tracer Overexpressed Protein Fluorescently Tagged Ligands Ectodermal Explants Animal Caps Ligand Diffusion Confocal Imaging Statistical Analysis Shape Of Ligand Gradients Morphogen Gradient Regulation
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