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Studying Wnt Signaling During Patterning of Conducting Airways
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Studying Wnt Signaling During Patterning of Conducting Airways

Studying Wnt Signaling During Patterning of Conducting Airways

DOI:

13:00 min

October 16, 2016

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Chapters

  • 00:05Title
  • 01:20Whole Mount X-galactosidase Staining
  • 05:23Lectin Staining
  • 06:18Whole Mount Immunofluorescence Staining and Confocal Microscopy
  • 07:43Cell Proliferation
  • 09:37Results: Wnt Signaling and Developing Tracheal and Lung Tissue
  • 11:28Conclusion

Summary

自动翻译

The use of reporter mice coupled to whole mount and section staining, microscopy and in vivo assays facilitates the analysis of mechanisms underlying the normal patterning of the respiratory tract. Here we describe how these techniques contributed to the analysis of Wnt signaling during tracheal development.

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