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Functional Cloning Using a Xenopus Oocyte Expression System
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发育生物学
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JoVE 杂志 发育生物学
Functional Cloning Using a Xenopus Oocyte Expression System

Functional Cloning Using a Xenopus Oocyte Expression System

7,868 Views

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09:40 min

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January 30, 2016

DOI:

10.3791/53518-v

DOI:
10.3791/53518-v

•

09:40 min
January 30, 2016

•

4 Views
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1Department of Biology,Shepherd University, 2Department of Biology,University of Virginia

Summary

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We describe a Xenopus oocyte and animal cap system for the expression cloning of genes capable of inducing a response in competent ectoderm, and discuss techniques for the subsequent analysis of such genes. This system is useful in the functional identification of a wide range of gene products.

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Cite this Article

Plautz, C. Z., Williams, H. C., Grainger, R. M. Functional Cloning Using a Xenopus Oocyte Expression System. J. Vis. Exp. (107), e53518, doi:10.3791/53518 (2016).

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