The manuscript here provides a simple set of methods for analysing the secretion and diffusion of fluorescently tagged ligands in Xenopus. This provides a context for testing the ability of other proteins to modify ligand distribution and allowing experiments that may give insight into mechanisms regulating morphogen gradients.
Fellgett, S. W., Ramsbottom, S. A., Maguire, R. J., Cross, S., O'Toole, P., Pownall, M. E. Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients. J. Vis. Exp. (106), e53162, doi:10.3791/53162 (2015).