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Chapters
- 00:05Title
- 01:06Cell Isolation and Culture
- 04:52Staining and Cell Sorting
- 08:37Results: Isolation of Human Adipose-derived Stromal Cells with Increased Osteogenic Potential
- 10:30Conclusion
The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.
