Journal
/
发育生物学
/
大规模的斑马鱼胚胎的心脏解剖的转录分析
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
Please note that all translations are automatically generated. Click here for the English version.

大规模的斑马鱼胚胎的心脏解剖的转录分析

13,973 Views

•

10:00 min

•

January 12, 2015

DOI:

10.3791/52087-v

DOI:
10.3791/52087-v

•

10:00 min
January 12, 2015

•

3 Views
, ,
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology,University of Potsdam, 3Institute of Molecular Biology,Medizinische Hochschule Hannover

Summary

Automatically generated

到斑马鱼心脏发育过程中分析的心脏基因表达分布，总RNA已被提取从离体心脏。在这里，我们提出了一个协议，用于收集功能/跳动的心迅速人工清扫，从斑马鱼胚胎获得心脏特异性mRNA。

Explore More Videos

ZebrafishEmbryonic HeartDissectionTranscriptional AnalysisCardiac TranscriptomeRNA ExtractionQuantitative Real-time PCRFluorescent LabelingFluidic Shear ForceFiltration

Read Article

Cite this Article

Lombardo, V. A., Otten, C., Abdelilah-Seyfried, S. Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis. J. Vis. Exp. (95), e52087, doi:10.3791/52087 (2015).

Download .ris file

Copy

Share Video

.

Copy