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Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using “Cargo Mapping” Analysis
1Department of Molecular and Experimental Medicine, Dorris Neuroscience Center,The Scripps Research Institute, 2Department of Cellular and Molecular Medicine,University of California San Diego, 3Department of Bioengineering,University of California San Diego, 4Department of Neurosciences,University of California San Diego School of Medicine
Chapters
- 00:05Title
- 02:37Preparation of Mouse Hippocampal Primary Neurons
- 03:46Live Imaging of Cargo Movement
- 06:20Cargo Mapping
- 08:35Results: ‘Cargo Mapping’ Determines the Motor Composition of Individual Moving Vesicles in Neurons
- 10:14Conclusion
Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.
