/
/
Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using “Cargo Mapping” Analysis
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using “Cargo Mapping” Analysis
9,252 Views
•
11:09 min
•
October 30, 2014
DOI:
1Department of Molecular and Experimental Medicine, Dorris Neuroscience Center,The Scripps Research Institute, 2Department of Cellular and Molecular Medicine,University of California San Diego, 3Department of Bioengineering,University of California San Diego, 4Department of Neurosciences,University of California San Diego School of Medicine
Summary
Automatically generated
Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.
Read Article
Cite this Article
Neumann, S., Campbell, G. E., Szpankowski, L., Goldstein, L. S., Encalada, S. E. Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis. J. Vis. Exp. (92), e52029, doi:10.3791/52029 (2014).
Copy