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사용 축삭화물을 이동하는 분자 모터의 구성을 특성화 "화물 매핑"분석
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11:09 min
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October 30, 2014
DOI:
1Department of Molecular and Experimental Medicine, Dorris Neuroscience Center,The Scripps Research Institute, 2Department of Cellular and Molecular Medicine,University of California San Diego, 3Department of Bioengineering,University of California San Diego, 4Department of Neurosciences,University of California San Diego School of Medicine
Summary
Automatically generated
Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.
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Cite this Article
Neumann, S., Campbell, G. E., Szpankowski, L., Goldstein, L. S., Encalada, S. E. Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis. J. Vis. Exp. (92), e52029, doi:10.3791/52029 (2014).
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