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初级量子神经元轴突运输的实时成像点标记的BDNF
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神经科学
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JoVE 杂志 神经科学
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
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初级量子神经元轴突运输的实时成像点标记的BDNF

14,904 Views

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10:53 min

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September 15, 2014

DOI:

10.3791/51899-v

DOI:
10.3791/51899-v

•

10:53 min
September 15, 2014

•

1 Views
, , , , ,
1Department of Neurosciences,University of California, San Diego, 2School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University, 3Department of Anesthesiology,University of California, San Diego, 4VA San Diego Healthcare System

Summary

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BDNF，神经营养因子，对轴突运输是几个神经元群体的生存和功能是至关重要的。一些退行性疾病的标志是轴索结构和功能的破坏。我们展示了用于检查QD-BDNF在使用初级神经元微流控室现场贩卖的技术。

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Cite this Article

Zhao, X., Zhou, Y., Weissmiller, A. M., Pearn, M. L., Mobley, W. C., Wu, C. Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons. J. Vis. Exp. (91), e51899, doi:10.3791/51899 (2014).

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