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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
 

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Article DOI: 10.3791/51554-v • 09:32 min • September 9th, 2014
September 9th, 2014 •
1, 1, 1, 2, 1, 1, 1
1Department of Hematology and Oncology, University Medical Center Göttingen, 2Microbial Interface Biology Group, Research Center Borstel

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We present a simple and efficient protocol for the generation of human macrophages. Buffy coats are processed by double density gradient centrifugation and isolated monocytes are then differentiated to macrophages in Teflon-coated cell culture bags. This maximizes macrophage yields and facilitates cell harvesting for subsequent experiments.

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Isolation Human Monocytes Double Gradient Centrifugation Differentiation Macrophages Teflon-coated Cell Culture Bags Pathologic Processes Infectious Diseases Cancer Understanding Mechanisms Isolation Protocol Buffy Coats Macrophage Yields Lab Equipment Cost-effective Time-effective Large Quantities Fluorinated Ethylene Propylene (FEP) Macrophage Colony-stimulating Factor (M-CSF) Functional Assays Quality Control Validation Disease Models
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