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Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
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JoVE 杂志 神经科学
Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices

DOI:
10.3791/50034-v

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10:04 min

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October 29, 2012

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, ,
1Department of Medicinal Chemistry and Molecular Pharmacology,Purdue University

Chapters

  • 00:05Title
  • 01:46Acute Brain Slice Preparation and Patch Clamp Recording
  • 03:47Local Application of Drugs to Neurons in Slices
  • 06:07Controlling the Drug-filled Micropipette with a Piezoelectric Translator
  • 07:22Results: Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
  • 09:33Conclusion

Summary

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自动翻译

In this paper, we describe a useful method to study ligand-gated ion channel function in neurons of acutely isolated brain slices. This method involves the use of a drug-filled micropipette for local application of drugs to neurons recorded using standard patch clamp techniques.

Tags

Keywords: Nicotinic Acetylcholine ReceptorNAChRNicotine AddictionElectrophysiologyWhole-cell Patch ClampBrain SlicesLocal Drug ApplicationNicotineAcetylcholineMouseNeuron

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