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培养成年的背根神经节神经元轴突再生的遗传研究

JoVE 杂志
神经科学

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JoVE 杂志神经科学

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

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培养成年的背根神经节神经元轴突再生的遗传研究

26,331 Views

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09:42 min

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August 17, 2012

DOI:

DOI:

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09:42 min

August 17, 2012

•

7 Views

Saijilafu¹, Feng-Quan Zhou^1,2

¹Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, ²Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Summary

一个<em>在体外</em用培养的成年小鼠背根神经节神经元的轴突再生的遗传研究模型描述。方法包括re-suspension/re-plating的步骤，允许进行遗传操作的神经元的轴突重新生长。这种做法是轴突再生功能丧失使用RNAi为基础的蛋白质击倒的研究特别有用。

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