需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Chapters
- 00:05Title
- 01:03Microdissection of the Cochlea
- 03:24Cell Dissociation
- 06:57Flow Cytometry
- 09:15Representative Results
- 10:52Conclusion
Flow cytometry is a powerful tool allowing for the isolation and study of specific cell populations. This protocol describes steps for isolating LacZ-expressing cells from cochlear tissues from neonatal transgenic mice. Dissociated cochlear cells were labeled using fluorescent-conjugated substrates of β-galactosidase prior to separation via flow cytometry.
Tags
Isolating LacZ-expressing CellsFlow CytometryRare Cell PopulationsStem/progenitor CellsTransgenic Reporter Mouse ModelsFluorescence-conjugated ProbesLacZ-positive CellsNeonatal CochleaeSensorineural Hearing LossCochlear Sensory Hair CellsRegenerative PotentialMurine Auditory And Vestibular OrgansStem/progenitor Cell CharacteristicsWnt Signaling PathwayAxin2-expressing CellsBehavior Of Wnt-responsive CellsAxin2-LacZ Reporter Mice
