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Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry
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Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry
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December 23, 2011
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Flow cytometry is a powerful tool allowing for the isolation and study of specific cell populations. This protocol describes steps for isolating LacZ-expressing cells from cochlear tissues from neonatal transgenic mice. Dissociated cochlear cells were labeled using fluorescent-conjugated substrates of β-galactosidase prior to separation via flow cytometry.
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Jan, T. A., Chai, R., Sayyid, Z. N., Cheng, A. G. Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry. J. Vis. Exp. (58), e3432, doi:10.3791/3432 (2011).
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