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Neuroscience

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Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry
 

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry

Article DOI: 10.3791/3432-v • 11:14 min • December 23rd, 2011
December 23rd, 2011 •
1, 1, 1, 1
1Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine

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Flow cytometry is a powerful tool allowing for the isolation and study of specific cell populations. This protocol describes steps for isolating LacZ-expressing cells from cochlear tissues from neonatal transgenic mice. Dissociated cochlear cells were labeled using fluorescent-conjugated substrates of β-galactosidase prior to separation via flow cytometry.

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Isolating LacZ-expressing Cells Mouse Inner Ear Tissues Flow Cytometry Rare Cell Populations Stem/progenitor Cells Inner Ear Tissues Transgenic Reporter Mouse Models Fluorescence-conjugated Probes LacZ-positive Cells Neonatal Cochleae Sensorineural Hearing Loss Cochlear Sensory Hair Cells Regenerative Potential Murine Auditory And Vestibular Organs Stem/progenitor Cell Characteristics Wnt Signaling Pathway Axin2-expressing Cells Behavior Of Wnt-responsive Cells Axin2-LacZ Reporter Mice
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