需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Isolando-LacZ expressar Células de tecidos de camundongos Inner Ear usando Citometria de Fluxo
11,049 Views
•
11:14 min
•
December 23, 2011
DOI:
Summary
Automatically generated
Flow cytometry is a powerful tool allowing for the isolation and study of specific cell populations. This protocol describes steps for isolating LacZ-expressing cells from cochlear tissues from neonatal transgenic mice. Dissociated cochlear cells were labeled using fluorescent-conjugated substrates of β-galactosidase prior to separation via flow cytometry.
Explore More Videos
Isolating LacZ-expressing CellsFlow CytometryRare Cell PopulationsStem/progenitor CellsTransgenic Reporter Mouse ModelsFluorescence-conjugated ProbesLacZ-positive CellsNeonatal CochleaeSensorineural Hearing LossCochlear Sensory Hair CellsRegenerative PotentialMurine Auditory And Vestibular OrgansStem/progenitor Cell CharacteristicsWnt Signaling PathwayAxin2-expressing CellsBehavior Of Wnt-responsive CellsAxin2-LacZ Reporter Mice
Read Article
Cite this Article
Jan, T. A., Chai, R., Sayyid, Z. N., Cheng, A. G. Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry. J. Vis. Exp. (58), e3432, doi:10.3791/3432 (2011).
Copy