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Strategies for Expressing and Purifying Solute Carrier Transporters
September 29, 2023
用于结构和生化研究的人溶质载体的高通量表达和纯化
02:10 min
•
将溶质载体 （SLC） 克隆到 pHTBV1.1 Bacmid 中以实现高通量蛋白表达
01:12 min
•
纯化人溶质载体 （SLC） 以开发 SLC 靶向疗法
03:51 min
•
Sagar Raturi
,
Huanyu Li
,
Yung-Ning Chang
,
Andreea Scacioc
,
Tina Bohstedt
,
Alejandra Fernandez-Cid
,
Adam Evans
,
Patrizia Abrusci
,
Abilasha Balakrishnan
,
Tomas C. Pascoa
,
Didi He
,
Gamma Chi
,
Nanki Kaur Singh
,
Mingda Ye
,
Anna Li
,
Leela Shrestha
,
Dong Wang
,
Eleanor P. Williams
,
Nicola A. Burgess-Brown
,
Katharina L. Dürr
,
Vera Puetter
,
Alvaro Ingles-Prieto
,
David B. Sauer
1
Centre for Medicines Discovery, Nuffield Department of Medicine
,
University of Oxford
,
2
Nuvisan ICB GmbH
,
3
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
Summary
Automatic Translation
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人膜转运蛋白的结构和生化研究需要毫克量的稳定、完整和均匀的蛋白质。在这里，我们描述了使用密码子优化基因筛选、表达和纯化人类溶质载体转运蛋白的可扩展方法。
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