Strategies for Expressing and Purifying Solute Carrier Transporters

September 29, 2023

Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes (Video) | JoVE

02:10 min

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Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression (Video) | JoVE

01:12 min

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Purification of Human Solute Carriers (SLCs) for Developing SLC Targeting Therapies (Video) | JoVE

03:51 min

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Sagar Raturi, Huanyu Li, Yung-Ning Chang, Andreea Scacioc, Tina Bohstedt, Alejandra Fernandez-Cid, Adam Evans, Patrizia Abrusci, Abilasha Balakrishnan, Tomas C. Pascoa, Didi He, Gamma Chi, Nanki Kaur Singh, Mingda Ye, Anna Li, Leela Shrestha, Dong Wang, Eleanor P. Williams, Nicola A. Burgess-Brown, Katharina L. Dürr, Vera Puetter, Alvaro Ingles-Prieto, David B. Sauer

¹Centre for Medicines Discovery, Nuffield Department of Medicine,University of Oxford, ²Nuvisan ICB GmbH, ³CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences

Summary

Structural and biochemical studies of human membrane transporters require milligram quantities of stable, intact, and homogeneous protein. Here we describe scalable methods to screen, express, and purify human solute carrier transporters using codon-optimized genes.

Strategies for Expressing and Purifying Solute Carrier Transporters

Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes (Video) | JoVE

Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression (Video) | JoVE

Purification of Human Solute Carriers (SLCs) for Developing SLC Targeting Therapies (Video) | JoVE

Summary

Article