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Journal
Biology
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
April 07, 2023
オルガノイド研究のためのマウス腸陰窩および単一幹細胞からのオルガノイドの3D培養
02:35 min
•
3,294 Views
陰窩分離のためのマウス小腸の処理
01:35 min
•
1,098 Views
マウス小腸からの陰窩分離
05:06 min
•
1,143 Views
単一腸管幹細胞からのオルガノイド培養
02:05 min
•
968 Views
Yuta Takase
1
,
Kazuto Fujishima
2,3
,
Toshio Takahashi
1
1
Suntory Foundation for Life Sciences
,
Bioorganic Research Institute
,
2
Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS)
,
Kyoto University
,
3
Faculty of Medicine
,
Osaka Medical and Pharmaceutical University
Summary
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Automatically Generated
マウス小腸陰窩を単離し、陰窩から腸3Dオルガノイドを培養するためのプロトコルについて説明します。さらに、上皮下細胞ニッチの非存在下で単一の腸幹細胞からオルガノイドを生成する方法について述べる。
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