The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

Yuta Takase; Kazuto Fujishima; Toshio Takahashi

doi:10.3791/65219

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3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

April 07, 2023

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

02:35 min

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3,296 Views

Processing Murine Small Intestine for Crypt Isolation

01:35 min

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Crypt Isolation from Murine Small Intestine

05:06 min

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Organoid Culture from a Single Intestinal Stem Cell

02:05 min

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Yuta Takase¹, Kazuto Fujishima^2,3, Toshio Takahashi¹

¹Suntory Foundation for Life Sciences,Bioorganic Research Institute, ²Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS),Kyoto University, ³Faculty of Medicine,Osaka Medical and Pharmaceutical University

Özet

We describe a protocol to isolate murine small intestinal crypts and culture intestinal 3D organoids from the crypts. Additionally, we describe a method to generate organoids from a single intestinal stem cell in the absence of a sub-epithelial cellular niche.