ジャーナル
生物学
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

April 07, 2023

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

02:35 min
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3,315 Views

Processing Murine Small Intestine for Crypt Isolation

01:35 min
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1,106 Views

Crypt Isolation from Murine Small Intestine

05:06 min
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1,153 Views

Organoid Culture from a Single Intestinal Stem Cell

02:05 min
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970 Views
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1Suntory Foundation for Life Sciences,Bioorganic Research Institute, 2Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS),Kyoto University, 3Faculty of Medicine,Osaka Medical and Pharmaceutical University

概要

We describe a protocol to isolate murine small intestinal crypts and culture intestinal 3D organoids from the crypts. Additionally, we describe a method to generate organoids from a single intestinal stem cell in the absence of a sub-epithelial cellular niche.

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