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Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
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Medicine
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JoVE Journal Medicine
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

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4,400 Views

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06:27 min

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May 13, 2021

DOI:

10.3791/62514-v

DOI:
10.3791/62514-v

•

06:27 min
May 13, 2021

•

14 Views
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1Department of Cardiology, Bejing Anzhen Hospital,Capital Medical University, 2Department of Cardiology,Bejing Chuiyangliu Hospital

Summary

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This protocol describes modifications of the Langendorff method including the depth of aorta cannulation for simultaneous Isolation of atrial and ventricular myocytes from adult mice.

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Wu, K., Li, L., Li, Y., Peng, X., Zhang, M., Liu, K., Wang, X., Yang, J., Wen, S., Ruan, Y., Liu, N., Bai, R. Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice. J. Vis. Exp. (171), e62514, doi:10.3791/62514 (2021).

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