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Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
Journal JoVE
Médecine
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Journal JoVE Médecine
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

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06:27 min

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May 13, 2021

DOI:

10.3791/62514-v

DOI:
10.3791/62514-v

•

06:27 min
May 13, 2021

•

14 Views
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1Department of Cardiology, Bejing Anzhen Hospital,Capital Medical University, 2Department of Cardiology,Bejing Chuiyangliu Hospital

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This protocol describes modifications of the Langendorff method including the depth of aorta cannulation for simultaneous Isolation of atrial and ventricular myocytes from adult mice.

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Langendorff MethodAtrial MyocytesVentricular MyocytesAdult MiceSimultaneous IsolationHeart DiseaseCellular MechanismsEuthanasiaAortic CannulationLangendorff ApparatusEnzymatic DigestionCell Isolation

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Wu, K., Li, L., Li, Y., Peng, X., Zhang, M., Liu, K., Wang, X., Yang, J., Wen, S., Ruan, Y., Liu, N., Bai, R. Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice. J. Vis. Exp. (171), e62514, doi:10.3791/62514 (2021).

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