Journal
/
Medicina
/
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
JoVE Revista
Medicina
This content is Free Access.
JoVE Revista Medicina
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

DOI:
10.3791/62514-v

•

06:27 min

•

May 13, 2021

•

, , , , , , , , , , ,
1Department of Cardiology, Bejing Anzhen Hospital,Capital Medical University, 2Department of Cardiology,Bejing Chuiyangliu Hospital

Capítulos

  • 00:05Introduction
  • 00:38Heart Excision and Aortic Cannulation
  • 02:38Heart Perfusion
  • 03:37Cell Isolation and Calcium Reintroduction
  • 04:46Results: Survival Rates and Quality of the Isolated Cardiac Myocytes
  • 06:00Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol describes modifications of the Langendorff method including the depth of aorta cannulation for simultaneous Isolation of atrial and ventricular myocytes from adult mice.

Tags

Keywords: Langendorff MethodAtrial MyocytesVentricular MyocytesAdult MiceSimultaneous IsolationHeart DiseaseCellular MechanismsEuthanasiaAortic CannulationLangendorff ApparatusEnzymatic DigestionCell Isolation

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/62514?article_type=v

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
check_url/es/62514?article_type=v

The WATCHMAN Left Atrial Appendage Closure Device for Atrial Fibrillation
check_url/es/62514?article_type=v

Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation
check_url/es/62514?article_type=v

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
check_url/es/62514?article_type=v

An Improved Method for Rapid Intubation of the Trachea in Mice
check_url/es/62514?article_type=v

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy
check_url/es/62514?article_type=v

Isolation and Cultivation of Adult Rat Cardiomyocytes
check_url/es/62514?article_type=v

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction
check_url/es/62514?article_type=v

Isolation of Atrial Myocytes from Adult Mice
check_url/es/62514?article_type=v

Invasive Hemodynamic Assessment for the Right Ventricular System and Hypoxia-Induced Pulmonary Arterial Hypertension in Mice

Read Article