Journal
/
Neuroscience
/
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

3,381 Views

•

06:21 min

•

October 06, 2020

DOI:

10.3791/61662-v

DOI:
10.3791/61662-v

•

06:21 min
October 06, 2020

•

3 Views
, ,
1Brudnick Neuropsychiatric Research Institute, Department of Neurobiology,University of Massachusetts Medical School, 2Interdisciplinary Graduate Program,University of Massachusetts Medical School, 3UMMS MD/PhD Program,University of Massachusetts Medical School

Tags

Single-cell ElectroporationOrganotypic Slice CultureMouse HippocampusExcitatory NeuronsInhibitory NeuronsGene TransfectionNeurobiologyElectroporation ProtocolHigh Transfection EfficiencyCell-autonomous MechanismsTranssynaptic Protein InteractionsSlice Culture PreparationElectroporator SetupPipette PreparationSlice Culture IsolationTarget Cell Electroporation

Read Article

Cite this Article

Keener, D. G., Cheung, A., Futai, K. Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons. J. Vis. Exp. (164), e61662, doi:10.3791/61662 (2020).

Download .ris file

Copy

Share Video

.

Copy